Eppendorf

Eppendorf^™ is a leading life science company that develops instruments, consumables and services for handling liquids, samples and cells in laboratories worldwide – with the commitment to deliver the best solution for managing your most precious research. Because, after 75 years, the mission of the company is just as valid and relevant as it ever was: “Eppendorf contributes to improving human living conditions.” So, since 1945, and for tomorrow as well, join the Eppendorf family and choose products that are a perfect fit for all your day-to-day laboratory work.

About Eppendorf

The Eppendorf product range includes pipettes and automated pipetting systems, dispensers, centrifuges, mixers, spectrometers and DNA amplification equipment, as well as ultra-low temperature freezers, fermenters, bioreactors, CO₂ incubators, shakers and cell manipulation systems. Consumables such as pipette tips, test tubes, microtitre plates and single-use bioreactor vessels complement the range of highest-quality premium products.

Eppendorf products are used widely in academic and commercial research laboratories, especially within the pharmaceutical, biotechnological, chemical and food sectors. The Eppendorf range is also perfect for clinical, forensic and environmental laboratories, and also the wider industrial environment wherever process analysis, production, and quality assurance protocols are being performed.

Accuracy, reliability, experience, innovation - characteristics that customers all over the world associate with Eppendorf.

Eppendorf's broad product range covers three competence areas:

Liquid Handling

Liquid handling is a core process in practically every life science laboratory. Naturally, this is one of Eppendorf's core areas of expertise. It's hard to imagine a laboratory without our completely reliable devices and consumables.

Cell Handling

From manipulation to cultivation - in microbiology and cell culture - more and more scientists from research to production have been using our cell handling products.

Sample Handling

As a scientist, your samples are your most valuable assets. Eppendorf knows this. Ensuring their safe handling and developing innovative solutions for a wide variety of sample processing requirements is what drives us.

Videos

Eppendorf 75 Years

We don’t know what will come. But we know that you will find out. Together we can look towards a future that inspires and improves people´s living conditions.

From Harvesting to Cell Manipulation

Are you familiar with the varied selection of Eppendorf products for applications in the modern cell biology laboratory?

ThermoMixer-Take Advantage

No matter your lab or applications, the Eppendorf ThermoMixer family integrates into your workflow with ease. Improve your assay results by mixing and incubating your samples at the same time – with highest vessel flexibility.

Get to know our customers

Transforming science for tomorrow: Watch how the University of East London keeps their position as one of the most modern universities of the UK - with Eppendorf at their side.

Molecular Biology – Life in Process

Are you familiar with the varied selection of Eppendorf products for applications in the modern molecular biology laboratory?

Precise and Repeatable Pipetting of Problem Liquids

Pipetting seems easy, but some tips and tricks can help you to get consistent and reproducible results - even with difficult liquids.

Reproducible DNA Amplification in PCR

Nowadays, PCR can be robust and easy—but you can still run into difficulties. Make sure to get the most out of your PCR with some simple tricks.

Aerosols in the Lab

Aerosols are colloidal systems of droplets and / or solid particles suspended in air. Aerosols can be found almost everywhere in the world and we inhale them continuously, so we should differentiate between harmful and harmless aerosols. The most important aerosols in the laboratory environment are bio-aerosols.

Resources

Literature Resources

White Papers

Posters

Pharma Centrifuge Solutions

Therapeutic Antibodies

The use of monoclonal antibodies (mAb) has been accepted as an effective cure for the treatment of cancer and autoimmune diseases. Today antibodies dominate the biopharmaceutical market and their development and production using hybridoma cells is widely established. Rising time and cost pressures in combination with the demand for higher throughput make the development of therapeutic antibodies challenging.

Many factors have to be taken into account and alternative solutions are evaluated to battle challenges like sample protection, efficiency, and reproducibility. In addition, optimized ergonomics can reduce hands-on time, protect lab staff from work-associated injuries and improve overall performance.

Drug Discovery Workflow

The mAb drug discovery process includes several centrifugation steps with individual requirements for the used device. Eppendorf solutions of centrifuges, rotors and vessel adapters are based on protocol evaluation and customer interviews within mAb drug discovery community.

We offer a variety of comprehensive packages for R&D from our large centrifuge portfolio which best fit your needs - so you have the peace of mind to focus on the medicine of tomorrow.

Therapeutic Antibodies Workflow and Eppendorf Solutions

Solutions for Your mAb Challenges

Eppendorf Centrifuge Solutions

1. Molecular Biology Solution 1. Molecular Biology Solution

Centrifuge 5425 +Rotor FA-18x2-Kit

Target antigen production, sequencing sample preparation, and antibody humanization involve a great deal of repetitive molecular biology handling steps. This includes spin column based MiniPrep kits for nucleic acid purification, gel extraction, and PCR clean-up. Each protocol has multiple centrifugation steps interrupted by pipetting and vessel changes. Microcentrifuges have entered a new generation, where the completely newly developed Centrifuge 5425 in combination with kit rotor specifically designed for 1.5/2.0 mL spin column kits.

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2. High Speed Solution 2. High Speed Solution

Centrifuge 5910 R + Rotor FA-6x50

Lysate clarification in nucleic acid purification protocols for target antigen production and antibody humanization include high speed centrifugation runs with up to 20,000 x g. High numbers of temperature-sensitive samples with up to 50 mL need to be processed. Each protocol has multiple centrifugation steps interrupted by pipetting and vessel changes. Refrigerated benchtop Centrifuge 5910 R in combination with low-weight aluminum rotors offer advanced cooling performance for optimal sample protection and reproducibility.

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3. Big Volume Harvesting Solution 3. Big Volume Harvesting Solution

Centrifuge 5910 R + Rotor S-4xUniversal + 4 x 1l adapter + 4 x 1l bottle

The production of antibodies in cell lines and antigens in bacteria involve harvesting cells from large volume suspensions of several liters. To guarantee sample integrity, cooling is key in these process steps. Refrigerated benchtop Centrifuge 5910 R in combination with 4 liter swing-bucket Rotor S-4xUniversal offer advanced cooling performance for optimal sample protection and reproducibility. For improved sustainability and economic efficiency large-volume bottles are reusable, autoclavable, UV-resistant and dishwasher-proof.

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4. Versatility Solution 4. Versatility Solution

Centrifuge 5910 R + Rotor S-4xUniversal + 4 x 5/15/plate adapter + 4 x 50/250/plate adapter

Purifying peripheral blood mononuclear cells (PBMC), FACS/Flow cytometry and hybridoma technology include multiple centrifugation steps of temperature-sensitive samples in many different consumables. This requires great versatility and an advanced temperature management. In addition, PBMC isolation by Ficoll gradient centrifugation must be carried out with a minimum of vibration during deceleration (breaking) within reasonable time. Refrigerated benchtop Centrifuge 5910 R in combination with swing-bucket Rotor S-4xUniversal offer great versatile and an advanced cooling performance for optimal sample protection. For faster high-yield PBMC isolation, acceleration and deceleration ramps can be selected individually.

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5. High-Throughput Screening Solution 5. High-Throughput Screening Solution

Centrifuge 5920 R + Rotor S-4-1000 (plate/tube bucket) + 4 x plate carrier

High-throughput screening for the identification of antibody hits in culture supernatants of hybridoma clones is usually performed in microtiter plates (MTPs) with 96 or more wells. Various methods are required and all include multiple centrifugation steps of temperature-sensitive samples in MTPs. Refrigerated benchtop Centrifuge 5920 R in combination with swing-bucket Rotor S-4x1000 with plate/tube buckets offer super-high capacity of up to 28 MTPs per run and an advanced cooling performance for optimal sample protection.

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6. Low-Throughput Plate Solution 6. Low-Throughput Plate Solution

Centrifuge 5430 + Rotor A-2-MTP

For the selection and characterization of antibody leads low-throughput screening assays are performed in microtiter plates (MTPs). Various methods are required and all include multiple centrifugation steps. Centrifuge 5430 in combination with swing-bucket Rotor A-2-MTP offer an economical solution for two MTPs per run in a small footprint. Centrifuge 5430 also accommodate rotors for 15 mL and 50 mL conical tubes and high-capacity rotors for 1.5/2.0 mL tubes.

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7. Classic Cell Culture Solution 7. Classic Cell Culture Solution

Centrifuge 5810 + Rotor S-4-104 + 4 x 5/15 mL adapter + 4 x 50 mL adapter

In a final step of the mAb drug discovery workflow a stable cell line is established that can express high amounts of humanized monoclonal antibody long-term. Cultivation and selection of stable cell lines require multiple centrifugation steps. Centrifuge 5810 in combination with Rotor S-4-104 offer an economical solution with an excellent footprint to capacity ratio. This setup is ideal for standard cell culture applications including cell harvesting in 15 mL or 50 mL conical tubes.

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Discover Eppendorf Centrifuges in Videos

Since 1964 Eppendorf has been manufacturing high-quality centrifuges and, from 1991, its R&D and production facilities in Leipzig, Germany have played a major role in this ongoing success story. Modern production methods, professional warehousing, highly qualified employees and a strong commitment to sustainability provide the basis for state-of-the-art products with renowned Eppendorf quality.

Our 5910R Centrifuge supports your therapeutic antibody drug discovery workflow with three innovative universal adapters, which accommodate a selection of tubes, plates and bottles. This improves handling and saves time. Learn more about it in our video!

Discover the Flow

Screening assays, protein expression, cell culture and upscaling are the main tools utilised during mAb drug discovery. The diverse methodology involves various vessel formats, kit applications, cooled sample handling, high-throughput applications or cell handling. All equipment needs to meet user requirements to ensure continued efficiency, reliability, sample and user safety. So, we have tailored perfect centrifugation bundles to the specific requirements of each mAb workflow application!

Do you have less time but more samples? Antigen concentration, hybridoma development, flow cytometry, and PBMC isolation involve many centrifugation runs with diverse vessel formats and temperature-sensitive samples. Our solution helps you handle versatile samples trouble-free with universal rotors and adapters, optimal cooling for sample safety, and programmable settings for fast and reproducible operation.

Literature Resources

Your Daily Companions

Microcentrifuges
- Your Daily Companions (1.4 MB)
It's Your Turn

Centrifuge 5702 family
- It's Your Turn (1.9 MB)
Reliable Performance

Centrifuge 5804/5810 family
- Reliable Performance (4 MB)
The Next Benchmark

Centrifuge 5910 R & 5920 R
- The Next Benchmark (12.8 MB)

Products

Eppendorf™ Polypropylene DNA LoBind Polypropylene Microcentrifuge Tube

Maximize sample recovery of nucleic acids by significantly reducing sample-to-surface binding with these tubes. Eppendorf™ Polypropylene DNA LoBind Tubes are ideal for sample preparation and long-term storage. X250 DNA LoBind tube 0,5ml PCR clean

Eppendorf™ Eppendorf Tubes™ Conical Tubes

Use these tubes for the simple and safe processing of sample volumes up to 5.0mL. Eppendorf Tubes™ represent the perfect option for working with medium–sized sample volumes. X200 Eppendorf Tubes 5,0ml, Biopur

Eppendorf™ Polypropylene Graduated Microtubes

Use these tubes for safe handling of valuable and toxic samples as well as radioactive substances or DNA. Eppendorf™ Polypropylene Graduated Microtubes feature a hinged lid with a small hook that clips around the rim of the tube. x1000 Safe-Lock microtube 2ml, assorted colours

Eppendorf™ Polypropylene Protein LoBind Microcentrifuge Tube

Store peptide, antibody or virus samples in these LoBind microcentrifuge tubes. Eppendorf™ Polypropylene Protein LoBind Microcentrifuge Tubes are ideal for work in protein research, mass spectrometry and protein array sample preparation. x100 Protein LoBind tube 2ml, PCR clean

Eppendorf™ ThermoStat™ C

Use this temperature control device for heating and cooling almost any of your lab vessels. The Eppendorf ThermoStat™ C is the ideal device to accurately set and maintain temperatures. Thermostat C w/o thermoblock, 220-240V EU plug

Eppendorf™ 3810X Polypropylene Microcentrifuge Tube

Use these tubes for all applications in molecular biology, clinical chemistry and biochemistry. Eppendorf™ 3810X Polypropylene Microcentrifuge Tube offers comfort and dependability for sample preparation, centrifugation and storage. x1000 Microtube 3810x1.5ml green

Eppendorf™ Combitips advanced™ Standard Pipettor Tips

Use these tips for comfortable handling while preventing damage to gloves. Eppendorf Combitips advanced™ Standard Pipetter Tips are optimized to meet the increased requirements of a modern laboratory, and offered in three different purity grades to support any application. X100 Combitips advanced 0,5ml, purple, bagged/25

Eppendorf™ ThermoMixer™ C Accessory, Smartblock™ Thermoblock

Maintain accurate uniform temperatures with these thermoblocks. Eppendorf™ Smartblock™ Thermoblocks are compatible with Eppendorf ThermoMixer™ C, and support a broad range of applications. SmartBlock for micro and deepwell plates , w/lidMicrotest- and Deepwell-pates with lid

Eppendorf™ epTIPS™ Standard Pipettor Tips

Use these universal pipette tips with a wide variety of pipette models. Eppendorf™ epT.I.P.S.™ Standard Pipettor Tips feature optimal wetting properties, high transparency and special, certified purity grades. X1000 EP-TIPS STD 1000ul

Eppendorf™ Centrifuge 5810 Classic Cell Culture Package (EU)

The Eppendorf Centrifuge 5810/5810 R is the perfect day-to-day centrifuge for laboratories with medium-throughput requirements. It has an extremely compact footprint yet still offers the real versatility of being able to take both tube and plate formats. non-IVD Centrifuge 5810 G, 230V, EU-plug, w/rotorS-4-104 and 15/50ml adapters

Eppendorf ThermoMixer™ C

Improve your assay results by dry incubation and mixing of your samples simultaneously with this mixer. The Eppendorf ThermoMixer™ C is ideal for heating, cooling and mixing almost any of your lab vessels. ThermoMixer C w/o thermoblock, 220-240V EU plug

Eppendorf™ 96-Well U-Bottom PCR Microplates

x80 Microplate 96/U-PP, clear/white, PCR clean(VE=80 Platten)

Eppendorf™ PCR Clean Colorless Safe-Lock Centrifuge Tubes

Protect your samples with these centrifuge tubes. Eppendorf™ PCR Clean Colorless Safe-Lock Centrifuge Tubes feature a hinged Eppendorf Safe–Lock lid for outstanding protection against unintentional opening during incubation and storage. X500 Tubes safe lock PCR clean 0.5mL Eppendorf

Eppendorf™ Conical Tubes

Enable easy opening and closing during one-handed operation. Allowing very tight closure, sample loss is minimized making them the ideal solution for cell culture and cell biology applications, and sample preparation in microbiology and molecular biology. x200 Conical Centrifuge Tube 15ml, PP, Amber, Sterile, bagged/50

Eppendorf™ UVette™

Use these cuvettes for measuring small volumes (≥50μL). The Eppendorf™ UVette™ is a disposable UV/Vis cuvette with a light transmission of 220 to 1,600nm. UVettes offer superb purity, convenience and precision for photometric analysis. x80 UVette

Eppendorf™ 2000μL Sterile Polypropylene 96-Well Deep Well PCR Plates

PCR plates, free of DNA, DNase, RNase and PCR inhibitors x80 Deepwell PP 96/2000ul, barcoded, steriledef. Farbe (Wells farblos), VE=80

Eppendorf™ Polypropylene PCR Tubes

Ensure efficient and homogenous heat transfer with these thin-walled polypropylene tubes. Eppendorf™ Polypropylene PCR Tubes are easy to open, but provide tight sealing to prevent evaporation in PCR. X120 PCR 0.2ml 8-Tubes Strip, PCR Clean, bagged/10(960 tubes) - individual hinged lid

Multipette™ E3 Single Channel Pipet

Dispenses and pipets any liquid regardless of viscosity while maintaining the highest standards for accurate results. Eppendorf™ Multipette™ E3 Single Channel Pipet provides full volume control, improved accuracy, and reproducibility while reducing physical strain during use. Multipette E3, with universal charging adapter and 2 Combitips advanced assortment packs

Eppendorf™ Combitips Advanced™ Biopur™ Pipette Tips

Meet the increased requirements of a modern laboratory with these pipetter tips. Eppendorf™ Combitips Advanced™ Biopur™ Pipetter Tips make working with radioactive, aggressive or toxic materials safer. Hermetically sealed piston prevents aerosol contamination. X100 Combitips advanced 0,2ml, light blue, Biopurindividually wrapped

Eppendorf™ Safe-Lock Tubes

The hinged lid of Eppendorf Safe-Lock Tubes offers outstanding protection against unintentional opening, and completely eliminates any danger of leaks during incubation and storage. x1000 Safe-Lock micro test tubes, 2ml, green

Eppendorf™ Eppendorf Tubes™ with Screw Cap

This product represents the perfect option for working with medium–sized sample volumes. It fills the gap between existing tube formats and enables the simple and safe processing of sample volumes up to 5.0 mL. X200 Tube 5ml, Amber, w/white screw cap, PCR clean, bagged/100

Eppendorf™ Xplorer™ Electronic Pipettes

Eliminate potential error risks associated with manual pipetting with these pipettes. Eppendorf™ Xplorer™ Electronic Pipettes provide precisely adjustable parameters, reproducible and accurate results. Experience fatigue-free pipetting and maintain full control over pipetting procedures. Xplorer, 8-channel, 5-100ul, yellow

Eppendorf™ ThermoMixer™ C Accessory: Smartblock™ Thermoblocks

Eppendorf™ Eppendorf Quality™ epT.I.P.S.™ Set

Eppendorf™ Eppendorf Quality™ epT.I.P.S.™ Set are the perfect combination that results in reduced-force tip attachment and ejection with complete sealing. x480 non-IVD epTIPS G set, 2-200ul, 53mm, yellow,96 tips/tray w/1 reusable box

Eppendorf™ Adapters for Rotors with 15mL Bore Holes

For centrifuges with rotors for conical 15 mL tubes X8 Universal insert Eppendorf 5mLfor rotors and adapters w/15mL conical bore holes

Multipette™ E3x Single Channel Pipet

Provides high-precision dispensing regardless of the physical properties of the liquid. Eppendorf™ Multipette™ E3x Single Channel Pipet allows one-handed operation and contact free tip ejection. Multipette E3x with universal charging adapter, charger stand, 2 Combitips advanced assortment packs

Eppendorf™ Storage Boxes

Allows selection of the optimal box combinations for individual storage demands. Eppendorf™ Storage Boxes provide safe storage for your sample tubes in a space-saving design. It offers a selection for tubes from 0.5mL to 50mL. X3 Storage Box PP for 81 (9x9) cryotubes 1-2mlH. 52.8mm

Eppendorf™ MixMate™ Basic Device (without tube holders

This 3-in-1 mixer for plates, tubes and vortex mixing is for where straight mixing is required. This high speed instrument provides complete mixing of your samples in seconds. Outstanding 2DMix-Control for optimized mixing with anti-spill technology. MixMate, 230V EU-Plug, without tube holder

Eppendorf™ Centrifuge MiniSpin™ plus

Use these entry-level centrifuges for a variety of general lab applications. Eppendorf™ Centrifuge MiniSpin™ plus features automatic lid opening at the end of the run to prevent sample warming and allow easy access to samples. CE-IVD Centrifuge Minispin Plus, EU-plug, with 12x2ml rotor

Eppendorf Microcentrifuge 5430 G with Keypad

Versatile general laboratory microcentrifuge that accepts 8 different rotors. non-IVD Centrifuge 5430 G, 230V, EU-plug, w/30x2ml rotor

High Capacity Shaker

Increase Your Yield with the Innova S44i Shaker

Are you looking for a stackable incubator shaker for particularly high protein expression yields in bacteria or yeast to quickly reach your ambitious targets? The Innova S44i from Eppendorf offers high vessel capacity and can sustain high speeds and loads 24/7.

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Shake faster! Ready for current high yield expression protocols

Current microbiological DNA and protein expression protocols lead to optimised yields with specialised flasks at shaking speeds of up to 400 rpm (e.g. Ultra Yield flasks from Thomson Instrument). Compared to other shakers in the market, the Innova S44i can provide and sustain high speeds at an orbit of 2.5 cm (1 in) 24/7 with maximum platform loads. This makes the Innova S44i a real workhorse for protein production. You can further increase your yield per footprint by triple stacking the device.

Innova S44i

The next evolution in biological shakers

Shake more! Up to twice as many flasks as other shakers in the market

Platform, chamber, and the heavy duty Eppendorf X-drive of the Innova S44i are optimised for high vessel capacity and load – to maximise the throughput of your lab. If vessel capacity is crucial for you, compare the capacity of the Innova S44i with other shakers in the market. Of course, the Innova S44i is also compatible with online in-flask monitoring systems like CGQ (by aquila biolabs) or PreSens, and microplate-screening systems like the Duetz-System.