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Molecular Probes™ APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU
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Quantity:
60 Assays
Unit Size:
5mL
Description
In collaboration with Phoenix Flow Systems, we offer the APO™-BrdU TUNEL Assay Kit which provides all the materials necessary to label and detect the DNA strand breaks of apoptotic cells. When DNA strands are cleaved or nicked by nucleases, a large number of 3´hydroxyl ends are exposed. In the APO-BrdU assay, these ends are labeled with BrdUTP and terminal deoxynucleotidyl transferase (TdT) using the TUNEL technique. Once incorporated ino the DNA, BrdU is detected using a bright and photostable green-fluorescent Alexa Fluor™ 488 dye-labeled anti-BrdU antibody.
Specifications
Specifications
| Color | Green |
| Content And Storage | Contains positive control cells (5 mL) and negative control cells (5 mL) of a fixed human lymphoma cell line, 1 vial of TdT (45 μL), 1 vial of BrdUTP (480 μL), 1 vial of anti-BrdU Ms mAb Alexa Fluor™ 488 conjugate (350 μL), 1 bottle of PI/Rnase A staining buffer (30 mL), 1 vial of reaction buffer (0.6 mL), 1 bottle of wash buffer (120 mL) and 1 bottle of rinse buffer (120 mL). Store as directed. |
| Detection Method | Fluorescence |
| Format | Tube |
| Excitation/Emission | 490/525 |
| Flow Cytometer Laser Lines | 488 |
| For Use With (Application) | Flow Cytometry |
| For Use With (Equipment) | Fluorescence Microscope, Flow Cytometer |
| Label or Dye | Alexa Fluor™ 488 |
| Product Type | TUNEL Assay Kit |
| Show More |
Safety and Handling
Product Identifier
- APO-BrdU TUNEL Assay Kit, with Alexa Fluor 488 Anti-BrdU
- Danger
- Serious eye damage/eye irritation Category 2
- Flammable liquid Category 2
- H225-Highly flammable liquid and vapour.
- H319-Causes serious eye irritation.
- P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
- P233-Keep container tightly closed.
- P240-Ground/bond container and receiving equipment.
- P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
- P242-Use only non-sparking tools.
- P243-Take precautionary measures against static discharge.
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P337+P313-If eye irritation persists: Get medical advice/attention.
- P370+P378-In case of fire: Use dry sand, dry chemical or alcohol-resistant foam to extinguish.
- P403+P235-Store in a well-ventilated place. Keep cool.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : ethanol
For Research Use Only. Not for use in diagnostic procedures.
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