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Cytiva GSTPrep™ FF 16/10 Column

Product Code. 10555905 Shop All Cytiva Products
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Quantity:
1 x 20 mL
Unit Size:
Each
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Fast and easy one-step, scale-up purification of GST-tagged recombinant proteins

Glutathione Sepharose™ 4 Fast Flow is prepacked in 20mL GSTPrep columns for purification of GST-tagged proteins (as well as glutathione S-transferases and glutathione-dependent proteins). Mild elution conditions preserve protein antigenicity and function.

Features:

  • Binds approx. 200mg recombinant GST/column
  • Simple operation with pump or chromatography system (e.g., AKTA™)

GSTPrep FF 16/10 Prepacked Columns

  • Volume: 20mL
  • Dimensions: 1.6 x 10cm
  • Recommended flowrate (H2O at room temp): 1 to 10mL/min. (30 to 300cm/hr.)
  • Max. flowrate (H2O at room temp): 10mL/min. (300cm/hr.)
  • Max. pressure during operation (H2O at room temp): 1.5bar (0.15MPa, 22psi)
  • Hardware pressure limit: 5bar (0.5MPa, 73psi)
  • Storage/temperature: 20% ethanol/4° to 30°C

TRUSTED_SUSTAINABILITY

Specifications

Buffer 1M Acetate, pH 6, 0.1M NaOH, 70% Ethanol or 6M Guanidine Hydrochloride
Product Type GSTPrep Glutathione Sepharose 4 FF column
Bed Volume 20mL
Diameter (English) Inner 0.62 in.
Diameter (Metric) Inner 16mm
Quantity 1 x 20 mL
Height (English) 3.9 in.
Height (Metric) 100mm
Flow Rate <10mL/min.
Ligand Type Glutathione and 10 Carbon linker arm
Max. Pressure 1.5 bar
pH Range 3 to 12
Particle Size 90μm
Support Type 4% Highly Cross-linked Agarose
Content And Storage 4° to 30°C
Column Type Ion Exchange Column
For Use With (Application) Purification of Glutathione S-transferases and Glutathione-dependent Proteins
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Safety and Handling

compliance-icons
Product Identifier
  • GSTPrep FF 16/10
 Signal Word
  • Warning
 Hazard Category
  • Flammable liquid Category 3
 Hazard Statement
  • H226-Flammable liquid and vapour.
 Precautionary Statement
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : ethanol
  • UNKNOWN ACUTE-16.5 per cent of the mixture consists of component(s) of unknown acute toxicity.
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