Thermo Scientific™ Halt™ Phosphatase Inhibitor Single-Use Cocktail

Beschreibung

Preserve phosphorylation of proteins in cells and tissues with Thermo Scientific™ Halt Phosphatase Inhibitor Cocktail.

• Halt Phosphatase Inhibitor Cocktail protects phosphoproteins from serine/threonine phosphatases, as well as protein tyrosine phosphatases (PTPs) during cell lysis
• The cocktail contains a mixture of four inhibitors of broad specificity including sodium fluoride, sodium orthovanadate, sodium pyrophosphate, and beta-glycerophosphate
• Conveniently packaged in a 100X format, the cocktail is stable at 4°C and is suitable for use with any cell or tissue extract
• The cocktail preserves protein phosphorylation in immunoprecipitation and kinase assays
• Phosphorylation and dephosphorylation comprise a molecular on/off switch that regulates many key biological pathways within the cell including signal transduction, cell division, and apoptosis
• To effectively study phosphorylation activation states, one must inactivate phosphatases to prevent their uncontrolled activity upon cell lysis

Additional Features

• Devoid of protease inhibitors – To protect against both proteases and phosphatases, use Halt Protease and Phosphatase Inhibitor Cocktail (78420)
• Dialyzable – Either dialyze or desalt sample to effectively remove inhibitors from cell extracts before performing procedures that are discovered to be incompatible with cocktail components
• Supplied as a 100X concentrate – effective when used at a final concentration of 1X; however, samples that contain particularly high levels of phosphatase activity may require a final concentration of 2-3X
• Compatibility – compatible with most detergent-based cell lysis reagents, including all Thermo Scientific Pierce Cell Lysis Solutions
• All-in-one format – inhibits both serine-threonine phosphatases and protein tyrosine phosphatases
• Compatible with popular protein assays – works with standard protein assays, including BCA and coomassie (Bradford) assays
• Convenient storage – refrigerator storage (2 to 8°C) saves valuable space in the freezer and allows for immediate use