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  6. Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns

Cytiva HiPrep™ Sephacryl™ HR Prepacked Gel Filtration Columns

Product Code. p-4591249 Shop All Cytiva Products
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Flow Rate:
<0.5 mL/min.
<1.3 mL/min.
Bed Volume:
120 mL
320 mL
Diameter (Metric) Inner:
1.6cm
2.6cm
Unit Size:
Each
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

For reproducible protein purification, separating polysaccharides, macromolecules with extended structures and small plasmids

Aids in preparative separation of biomolecules from up to 5mL (for 16mm column I.D.) and 13mL (for 26mm column I.D.).

  • Provides maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability
  • Prepacked columns for use with AKTAdesign™ or other pumped chromatography systems
  • Available in two different prepacked column sizes - 16 x 600mm (120mL) and 26 x 60mm (320mL)
  • Maximum pressure (over packed bed during operation) is 1.5 bar (22psi)

Media    

  • Average particle size: 50μm
  • Matrix: Allyl dextran and N, N'-methylenebisacrylamide
  • pH Stability working range: 3 to 11
  • pH Stability cleaning range: 2 to 13
  • Chemical stability: all commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1% SDS, 20% ethanol, 30% propanol, 30% acetonitrile (tested at 40°C for one week), 0.5 M NaOH (only for cleaning-in-place)
  • Pressure/Flow specification: >125cm/hr., 100kPa, XK 50/30, bed height 15cm
Column
  • Material (bed support): Polymethyl-Methacrylate

HiPrep 16/60 Sephacryl S-400 HR

  • Media:
  • Fractionation [Mr] globular proteins: 2 x 104-8 x 106
  • Exclusion limit [Mr] (globular proteins): ≈9 x 106 (9000kDa)
  • Fractionation range [Mr] DNA exclusion limit:    271bp
  • Fractionation [Mr] Dextrans: 1 x 104-2 x 106
HiPrep 16/60 Sephacryl S-500 HR
  • Media:
  • Exclusion limit for Dextrans: 100 x 106
  • Fractionation range [Mr] DNA exclusion limit: 1078bp
  • Fractionation [Mr] Dextrans: 4 x 104-2 x 107

TRUSTED_SUSTAINABILITY

Specifications

Quantity Each
Flow Rate <1.3 mL/min.
Particle Size 25 to 75 μm
For Use With (Equipment) Low to medium pressure AKTA™ systems or pumped chromatography systems
Bed Volume 120 mL
Product Type Sephacryl HR Pre-packed Gel Filtration Column
Diameter (Metric) Inner 1.6cm
For Use With (Application) Reproducible Protein purification, separating Polysaccharides, macromolecules with extended structures and small plasmids
Height (English) 23.62 in.
Height (Metric) 60cm
Material Polypropylene
Max. Pressure 1.5 bar [0.15 MPa] (22 psi)
pH Range 3 to 11
Support Type Allyl Dextran and N,N'-methylenebisacrylamide
Sample Type Polysaccharides, macromolecules with extended structures and even small plasmids
Content And Storage 4°C to 30°C, 20% Ethanol
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Säkerhet och hantering

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Produktbeteckning
  • HiPrep 16/60 Sephacryl S-500 HR
 Signalord
  • Warning
 Riskkategorier
  • Flammable liquid Category 3
 Faroangivelser
  • H226-Flammable liquid and vapour.
 Skyddsangivelse
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P235-Keep cool.
  • P501-Dispose of contents/container to .
 Kompletterande information
  • MIXTURE LIST-Contains : ethanol
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