Invitrogen™ Click-IT™ ManNAz Metabolic Glycoprotein Labeling Reagent (tetraacetylated N-Azidoacetyl-D-Mannosamine)

The Click-iT ManNAz metabolic glycoprotein labeling reagent provides the first part of a simple and robust two-step technique to identify and characterize cell surface sialic acid-containing glycoproteins. In step one, cultured cells are incubated with the azide-modified mannosamine (ManNAz). The azido-sugar is metabolically incorporated into cell surface sialic acid-containing glycoproteins through the permissive nature of the oligosaccharide biosynthesis pathway. In step two, via the chemoselective ligation or click reaction between an azide and an alkyne, the azido-labeled glycoproteins can then be detected with a Click-iT Glycoprotein Detection kit for gels (TAMRA or Dapoxyl™ alkyne) or Western blots (biotin alkyne). These Click-iT products are compatible with LC-MS/MS and Multiplexed Proteomics™ technologies for in-depth analyses of the glycoproteome.

• Less hazardous

Cell Analysis, Click Chemistries, Glycoprotein Analysis with Click Chemistry, Labeling Chemistry, Mass Spectrometry, Metabolic Labeling, Peptide Labeling, Protein and Antibody Labeling, Protein Gel Electrophoresis, Protein Gel Staining and Imaging, Proteins, Expression, Isolation and Analysis