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Invitrogen™ Image-iT™ Fixation/Permeabilization Kit

Product Code. 12363643
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Quantity:
1 kit
Unit Size:
Each
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Includes

All components are provided at 1X and are ready to use. Kit Contains: High purity 4% formaldehyde in PBS, pH 7.3; 0.5% Triton X-100; 3% BSA, fraction V, delipidated, New Zealand source, in DPBS; PBS, pH 7.4

Convenient-to-use fixation/permeabilization kit that contains all necessary reagents to prepare cells for antibody staining and imaging

  • Components help preserve cell morphology and reduce background staining and come in practical box with single-use vials and easy-to-follow protocols
  • Complete fixation/permeabilization kit with ready-to-use components and room temperature storage
  • Optimized for secondary antibody labeling, fixed-cell dye staining, and GFP applications
  • Convenient storage box, 1X ready-to-use components
  • Compatible with analysis of most cellular antigens
  • Reduced background staining
  • Components were chosen to meet wide range of sample preparation requirements demanded for successful fluorescent imaging of fixed and permeabilized cell
  • Reagents optimaized for both preservation of 3D cellular structure and adequate antibody access to intracellular antigenic sites
  • Kit components were extensively tested with both nuclear and cytoplasmic targets and showed equal or superior results compared to standard reagents
  • Cells are treated sequentially with fixative, permeabilization solution, and blocking buffer according to simplified and easy-to-follow protocol: This procedure affords optimal access to intracellular structures for antibodies and fixed cell dyes and maintains cellular morphology for subsequent fluorescent cell imaging
  • Decreased background staining, consequently improving signal-to-noise levels and image quality

Cell Analysis, Cellular Imaging, IHC Staining and Detection, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunofluorescence Sample Preparation, Immunohistochemistry (IHC)

Order Info

Shipping Condition: Room temperature

TRUSTED_SUSTAINABILITY

Specifications

Type Fixation/Permeabilization Kit
Product Line Image-iT Molecular Probes
Reagent Type Fixation Reagent, Permeabilization Reagent, Triton X-100
Content And Storage Fixative Solution: 20 mL
  • Permeabilization solution: 20 mL
  • Blocking solution: 12 x 10 mL
  • Wash Buffer: 500 mL
  • Store at ≤25 °C.
    		•
    Quantity 1 kit
    Shipping Condition Room Temperature
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