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Macherey-Nagel™ NucleoSpin™ RNA Plant, Mini kit for RNA from plant
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No. of Reactions:
10 Preps
250 Preps
50 Preps
Unit Size:
10 reactions
250 reactions
50 reactions
Description
NucleoSpin™ RNA Plant Kit is designed for isolation of DNA-free, high-quality RNA from a wide variety of plant and fungal samples. Typical downstream applications include real-time RT-PCR, gene expression profiling, Northern blotting, primer extension, array technology, and RNase protection assays.
- Two different lysis buffers included—Optimized lysis procedure for typical conditions and for peculiar metabolites
- rDNase included for on-column digestion—Efficient removal of contaminating DNA
- NucleoSpin™ Filters (shredders) included—Efficient sample homogenization and reduction of viscosity
- Parallel purification of DNA possible—By using the NucleoSpin™ RNA/DNA buffer set
Kit Components: NucleoSpin™ RNA columns with 2 mL collection tubes, 2 mL and 1.5 mL collecting tubes, NucleoSpin™ filters, buffers (lysis, wash, desalting), RNase-free DNase I, reaction buffer for DNase, RNase-free water
- Mini spin column with silica membrane technology
- Yields 3 to 70μg RNA from 100μg plant material
- Elution volume: 40 to 60μL
- Fragment size: 200b–20kb
- Binding capacity: 200μg
- Preparation time: 30 min./6 preps
- A260/A280: 1.9–2.1
Specifications
Specifications
| Format | Mini Spin Column |
| For Use With (Equipment) | Real-time RT-PCR |
| For Use With (Application) | RNA purification from plant cells and tissue |
| Includes | Lysis buffer RA1 (10mL), Lysis buffer RAP (10mL), Wash buffer RAW2 (13mL), Wash Buffer RA3 concentrate (6mL), Membrane desalting buffer (10mL), Reaction buffer for rDNase (7mL), RNase-free rDNase (1 size D vial), RNase-free water (13mL), NucleoSpin filters (10), NucleoSpin RNA Plan Columns with collection tubes (10), 2mL Collection tubes (30), 1.5mL Collection tubes (10) |
| No. of Reactions | 10 Preps |
| Product Type | NucleoSpin™ RNA Plant Column |
| Quantity | 1 Each |
| Sample Size | <100 mg |
| Yield | 3–70μg |
Safety and Handling
Signal Word
- Danger
- Flammable liquid Category 3
- Acute toxicity Category 4
- Skin corrosion/irritation Category 2
- Serious eye damage/eye irritation Category 2
- Respiratory sensitiser Category 1
- LONG-TERM AQUATIC HAZARD Chronic 3
- H226-Flammable liquid and vapour.
- H302-Harmful if swallowed.
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H334-May cause allergy or asthma symptoms or breathing difficulties if inhaled.
- H412-Harmful to aquatic life with long lasting effects.
- P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
- P342+P311-If experiencing respiratory symptoms: Call a POISON CENTER/doctor/ .
- MIXTURE LIST-Contains : ethanol (diluted < 20 %)(denatured with1%IPA/1%MEK, acc.2016/1867/EU); guanidinium thiocyanate; gua
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