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Thermo Scientific™ EZ-Link™ Activated Peroxidase Antibody Labeling Kit

Product Code. 10035883 Shop All Thermo Scientific Products
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Quantity:
5 Reactions
Unit Size:
1 set
This item is not returnable. View return policy
Availability may vary by country
This item is not returnable. View return policy
Availability may vary by country

Description

Glutaraldehyde-activated horseradish peroxidase (HRP) enzyme and kit to label primary antibodies and purify the resulting antibody-HRP conjugates.

Thermo Scientific™ Pierce Activated HRP and Antibody Labeling Kit contains horseradish peroxidase enzyme that has been glutaraldehyde-activated to enable labeling and preparation of antibody conjugates.

This chemistry can be used to couple HRP to primary amines on another protein such as an antibody. The Thermo Scientific Activated Peroxidase Kit is designed to purify HRP-antibody conjugates by using Immobilized Protein A/G to separate unreacted enzyme from the antibody conjugate. Using Activated HRP produces an HRP-antibody conjugate in which both the enzyme activity and the antigen-binding activity are preserved.

Highlights:

  • Reacts with primary amines to form a covalent amide bond
  • No reduction step is necessary to secure the linkage
  • Can be stored for at least 12 months at -20'C
  • One mg produces about 0.5mL of conjugate with a working dilution of 1:1,000 when coupled to a high titer antibody
  • Enzyme activity is > 200 units/mg

Includes:

5mg Activated Peroxidase, 50mL Conjugation Buffer, 250mg Lysine, 0.5mL Immobilized Protein A/G Column, 200mL Gentle Ag/Ab Binding Buffer, 200mL Gentle Ag/Ab Elution Buffer

TRUSTED_SUSTAINABILITY

Specifications

Product Type Antibody Labeling Kit
Quantity 5 Reactions
Sufficient For 5 conjugations, each with 0.3 mg antibody (IgG)
Content And Storage Sufficient For: 5 conjugations, each with 0.3 mg antibody (IgG)
  • Activated Peroxidase, 5 mg
  • Conjugation Buffer, 50 mL
  • Lysine, 250 mg
  • Protein A/G Column, 0.5 mL
  • Gentle Ag/Ab Binding Buffer, 200 mL
  • Gentle Ag/Ab Elution Buffer, 200 mL

    Store activated peroxidase at -20°C. Store other components at 4°C.
    		•
    Labeling Scale 300 μg
    Product Line EZ-Link™
    Label or Dye HRP (Horseradish Peroxidase)
    Labeling Target IgG

    For Research Use Only. Not for use in diagnostic procedures.

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