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Thermo Scientific™ DTNB (Ellman's Reagent) (5,5-dithio-bis-(2-nitrobenzoic acid)

Product Code. 10086113 Shop All Thermo Scientific Products
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Quantity:
5 g
Unit Size:
5g
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Measure peptide cysteines or other free, reduced sulfhydryls (-SH groups) in solution using this thiol-reactive, colored compound (Amax 412nm).

Thermo Scientific™ Pierce DTNB (Ellman's Reagent) reacts with sulfhydryl groups to yield a colored product, which provides a reliable method for measure free reduced cysteines and other free sulfhydryls in solution.

A solution of this compound produces a measurable yellow-colored product when it reacts with sulfhydryls. Consequently, Ellman's Reagent is very useful as a sulfhydryl assay reagent because of its specificity for –SH groups at neutral pH, high molar extinction coefficient and short reaction time. Sulfhydryl groups may be estimated in a sample by comparison to a standard curve composed of known concentrations of a sulfhydryl-containing compound such as cysteine. Alternatively, sulfhydryl groups may be quantitated by reference to the extinction coefficient of TNB.

Highlights:

  • Sulfhydryl detection– reacts quantitatively with free (reduced) sulfhydryl groups

  • Colorimetric–yields colored product to enable spectrophotemetric measurement in tubes or microplates

  • Proven assay method– well-characterized chemistry enables quantitation of peptide or protein sulfhydryl groups using extinction coefficient calculation or by comparison to a cysteine standard

TRUSTED_SUSTAINABILITY

Specifications

Product Type DTNB
Quantity 5 g

Safety and Handling

compliance-icons
Product Identifier
  • DTNB (Ellman's Reagent) (5,5-dithio-bis-(2-nitrobenzoic acid)
 Signal Word
  • Warning
 Hazard Category
  • Serious eye damage/eye irritation Category 2
  • Skin corrosion/irritation Category 2
  • Specific target organ toxicity Category 3
 Hazard Statement
  • H315-Causes skin irritation.
  • H319-Causes serious eye irritation.
  • H335-May cause respiratory irritation.
 Precautionary Statement
  • P260-Do not breathe dust/fume/gas/mist/vapours/spray.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P310-Immediately call a POISON CENTER/doctor/.
  • P405-Store locked up.
  • P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
 Supplemental information
  • MIXTURE LIST-Contains : 3,3'-dithiobis [6-nitrobenzoic] acid

For Research Use Only. Not for use in diagnostic procedures.

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