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Thermo Scientific™ HABA (4'-hydroxyazobenzene-2-carboxylic acid)
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Thermo Scientific™ Pierce HABA is 4'-hydroxyazobenzene-2-carboxylic acid, a simple reagent that enables spectrophotometric (colorimetric) estimation of biotinylation levels of labeled proteins and other molecules.
Thermo Scientific Pierce HABA is 4'-hydroxyazobenzene-2-carboxylic acid, a simple reagent that enables spectrophotometric (colorimetric) estimation of biotinylation levels of labeled proteins and other molecules. Determine the molar ratio of biotin incorporated into a protein using the HABA-Avidin method. The HABA dye (4'-hydroxyazobenzene-2-carboxylic acid ) binds to avidin to produce a yellow-orange colored complex which absorbs at 500nm. Free biotin will displace the HABA dye and cause the absorbance to decrease. A standard curve can be established using the free biotin to estimate the number of moles of biotin incorporated after biotinylating a protein.
Highlights:
- HABA-avidin complex can be used over a wide range of pH and salt concentrations
- Amount of Avidin can be calculated directly from the increased absorbance at 500nm complexing with the HABA Dye
- Calculate results directly from absorbance values based on extinction coefficients using the procedure outlined in the instructions or use Pierce's online HABA calculator
- Complete kits also available! See Pierce Biotin Quantitation Kit (Part No. 28005) and Fluorescence Biotin Quantitation Kit (Part No. 46610)
Specifications
Specifications
| Product Type | HABA |
| Quantity | 10 g |
| Content And Storage | Upon receipt store product at room temperature. Product shipped at ambient temperature. |
| Product Line | Pierce™ |
| Label or Dye | Biotin |
| Labeling Target | Antibodies, Proteins |
Safety and Handling
- HABA (4'-hydroxyazobenzene-2-carboxylic acid)
- Warning
- Serious eye damage/eye irritation Category 2
- Skin corrosion/irritation Category 2
- Specific target organ toxicity Category 3
- H315-Causes skin irritation.
- H319-Causes serious eye irritation.
- H335-May cause respiratory irritation.
- P260-Do not breathe dust/fume/gas/mist/vapours/spray.
- P264-Wash hands thoroughly after handling.
- P280-Wear protective gloves/protective clothing/eye protection/face protection.
- P301+P330+P331-IF SWALLOWED: rinse mouth. Do NOT induce vomiting.
- P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
- P305+P351+P338-IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
- P405-Store locked up.
- P501b-Dispose of contents/container in accordance with local/regional/national/international regulations.
- MIXTURE LIST-Contains : 2-(p-hydroxyphenylazo)benzoic acid
For Research Use Only. Not for use in diagnostic procedures.
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