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  5. IBA Lifesciences pDSG-IBA Small Mammalian Expression Vector

IBA Lifesciences pDSG-IBA Small Mammalian Expression Vector
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Product Code. p-7139102 Shop All IBA Lifesciences Products
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Vector:
pDSG-IBA102
pDSG-IBA103
pDSG-IBA104
pDSG-IBA105
pDSG-IBA43
pDSG-IBA45
pDSG-IBAwt1
pDSG-IBAwt2
Protein Tag:
His Tag (6x), Strep-tag II
Twin-Strep-tag
Untagged
Unit Size:
5µg
This item is not returnable. View return policy
This item is not returnable. View return policy

Description

Designed for protein expression for mammalian HEK293E cells (MEXi 293E cells). IBA Lifesciences GmbH Small Mammalian Expression Vector is a small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system.

pDSG-IBA vectors for MEXi mammalian expression system

  • Small transient expression vector especially developed for the use in combination with the MEXi mammalian expression system.
  • Designed for high-level stable and non-replicative transient expression in most mammalian hosts containing the following elements:
    • Human cytomegalovirus (CMV) immediate-early promoter for high-level expression in a wide range of mammalian cells
    • Episomal replication through Epstein Barr Virus replication origin (oriP) requires chromosomally expressed nuclear antigen encoded by EBNA-1
    • Ampicillin resistance and ColE1 replication origin (pUC) for propagation in E. coliin a high plasmid copy number

Please note that cloning into pDSG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pDSG-IBA vectors with Esp3I, another option via a so-called Entry-Vector is possible.

Specifications

Constitutive or Inducible System Constitutive
Marker Gene Ampicillin
Promoter CMV
Product Type Vector
Selection Agent (Eukaryotic) Geneticin™ (G-418)
Content And Storage Recommended: 2°C to 8°C for frequent usage, -20°C for long-term storage
Species Mammalian
Protein Tag His Tag (6x), Strep-tag II
Cloning Method Direct Cloning
Concentration 250 ng/μL
Primer Forward
Quantity 5 μg
Vector pDSG-IBA45
For Use With (Application) Vector for recombinant expression in mammalian cells
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