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Thermo Scientific™ Pierce™ ECL 2 Western Blotting Substrate

Recommended HRP substrate for fluorescence imagers

4945.00 DKK

Specifications

Product Type ECL Western Blot Substrate
Kit Contents Detection reagent 1 and 2
Content And Storage 2°C to 8°C
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Includes: Detection Reagent 1 and 2

Products 1
Product Code Brand Quantity Sufficient For Price Quantity & Availability  
Product Code Brand Quantity Sufficient For Price Quantity & Availability  
11517371
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Thermo Scientific™
80196X3
3 x 100 mL 30 Mini-gel blots, 3000cm2 of membrane This item is currently unavailable or has been discontinued.
View the product page for possible alternatives.
N/A
Description

Description

Pierce ECL 2 Substrate is a chemiluminescent and chemifluorescent substrate to detect peroxidase activity from HRP-conjugated antibodies and probes by Western blotting using X-ray film, CCD camera documentation systems or laser-based imagers. Pierce ECL 2 Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers.

Pierce ECL 2 substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. A broad dynamic range and excellent sensitivity combined with a long-lasting signal make Pierce ECL 2 Substrate a desirable Western blotting substrate.

Highlights:

  • High sensitivity — detect targets down to the low-picogram level
  • Long signal duration — sustained light output for as long as five hours
  • More imaging options — X-ray, CCD or laser-based imagers
  • More affordable — high quality and performance at a lower price than other suppliers' similar substrates

Specifications

Specifications

ECL Western Blot Substrate
Detection reagent 1 and 2
2°C to 8°C
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Safety and Handling

Safety and Handling

Product Identifier
  • Pierce ECL 2 Western Blotting Substrate

Signal Word
  • Danger

Hazard Category
  • Flammable liquid Category 2
  • Serious eye damage/eye irritation Category 2
  • Carcinogenicity Category 2
  • Specific target organ toxicity Category 3

Hazard Statement
  • H225-Highly flammable liquid and vapour.
  • H319-Causes serious eye irritation.
  • H351-Suspected of causing cancer.
  • H335-May cause respiratory irritation.

Precautionary Statement
  • P201-Obtain special instructions before use.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P241-Use explosion-proof electrical/ventilating/lighting/ equipment.
  • P303+P361+P353-IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water/ shower.
  • P304+P340-IF INHALED: Remove person to fresh air and keep comfortable for breathing.
  • P235-Keep cool.
  • P501-Dispose of contents/container to .

Supplemental information
  • MIXTURE LIST-Contains : 1, 4-dioxane, ethanol

SDS
Documents

Documents

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