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Thermo Scientific™ Vivid™ Green Fluorescent Standard
Description
Vivid™ Fluorescent Standards represent the products formed during oxidation of Vivid™ Substrates by cytochrome P450 enzymes. These standards enable quantification of enzyme activity when used as part of the Vivid™ CYP450 Screening Kits. Vivid™ Fluorescent Standards are included in the Vivid™ Screening Kits, but may also be purchased separately. They are supplied as a dried film.
More About Vivid™ CYP450 Screening Kits
Vivid™ CYP450 Screening Kits are high-throughput fluorescence-based assays for detection of enzyme-drug interactions and CYP450 inhibition. Vivid™ CYP450 Screening Kits include Vivid™ Substrate, Vivid™ Fluorescent Standard, reaction buffer, Vivid™ Regeneration System, NADP+, and CYP450 BACULOSOMES™ Plus Reagent. Vivid™ Fluorogenic Substrates are blocked dyes that yield minimal fluorescence signal until cleaved or hydroxylated. Oxidation at either of two potential sites releases the highly fluorescent product. CYP450 BACULOSOMES™ Plus Reagents are microsomes prepared from insect cells expressing a human CYP450 isozyme and human cytochrome P450 reductase. CYP450 BACULOSOMES™ Plus Reagents offer a distinct advantage over human liver microsomes in that only one CYP450 isozyme is expressed, thereby preventing metabolism by other CYP450s.
Applications
- High-throughput screening of enzyme-drug interactions
- Compound profiling for drug inhibition of cytochrome P450 isozymes
- Generation of predictive SAR models to guide compound acquisition
Specifications
Specifications
| Description | Vivid™ Green Fluorescent Standard, Cell Viability, Proliferation and Function, Pharma and Biopharma, Protein Assays and Analysis, Target-Based ADME/Tox Assays, For Research Use Only, Not for Use in Diagnostic Procedures |
| For Use With (Application) | Cell viability, proliferation and function, pharma and biopharma, protein assays and analysis, target-based ADME/Tox assays |
| Quantity | 1 Each |
| Product Type | Fluorescent Standard |
| Kit Contents | Vivid™ CYP450 Screening kits include Vivid™ CYP450 fluorogenic substrates, BACULOSOMES™ CYP450 reagents, fluorescent standards, and an NADPH-regeneration system. Vivid™ CYP450 fluorogenic substrates are also available separately |
| Content And Storage | • Vivid™ CYP450 Fluorogenic substrates • BACULOSOMES™ CYP450 reagents • Fluorescent standards • NADPH-regeneration system. Vivid™ CYP450 Fluorogenic substrates are also available separately. Store reaction buffers at +4°C or room temperature. Store BACULOSOMES™, the NADPH-regeneration system, and NADP+ at -80°C. Store the Vivid™ substrates and standards at +4°C, protected from light. (For long-term storage, resuspend in organic solvent and keep at -20°C.) |
| Shipping Condition | Wet Ice |
| Label or Dye | Other Label(s) or Dye(s) |
| Product Line | VIVID™ |
For Research Use Only. Not for use in diagnostic procedures.
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